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The Battle of the PCRs How qPCR and dPCR Are Transforming Veterinary Health

14 Jan 2025

When it comes to protecting poultry from dangerous viral infections, early and accurate detection is key. Two powerful tools quantitative PCR and digital PCR are transforming how we identify these diseases. But what’s the difference between the two, and why is dPCRemerging as a game-changer? Let’s dive into the tech that’s reshaping poultry diagnostics and explore how vaccines can help prevent outbreaks.

According to BIS Research, the digital PCR market , valued at $678.1 million in 2023, is projected to grow at a robust CAGR of 15.75%, reaching an estimated $3,351.0 million by 2034.

Quantitative PCR: The Reliable Workhorse of Diagnostics

qPCR has been a trusted ally in veterinary medicine for years. It works by amplifying DNA and measuring it in real-time, making it quick and effective for identifying individual viruses. However, when multiple pathogens are present, qPCR struggles to provide the same level of accuracy. While great for single-target detection, it’s not always the best tool for complex co-infections.

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 Digital PCR: The Next Big Thing in Disease Detection

Enter digital PCR, the next-level diagnostic technology that’s changing the game. Unlike qPCR, dPCR breaks down DNA samples into thousands of tiny droplets and analyzes them individually. This approach delivers precise and absolute quantification of pathogens, making it perfect for identifying multiple infections at once.

For example, dPCR has proven its worth in detecting Duck Tembusu Virus (DTMUV), Duck Circovirus (DuCV), and New Duck Reovirus (NDRV)—three viruses that can devastate poultry populations. By detecting all three in a single test, dPCR saves time, reduces costs, and provides more accurate results than traditional methods.

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Vaccines: The Ultimate Shield Against Poultry Viruses

While diagnostics are essential for early detection, prevention is always better than cure. That’s where vaccines come in. Vaccines for DTMUV, DuCV, and NDRV are readily available and are vital for controlling these diseases. Regular vaccination programs, combined with strict biosecurity measures, create a solid defense against outbreaks.

Vaccinating ducks not only protects individual birds but also boosts overall flock immunity, ensuring healthier poultry and more sustainable farming. When paired with advanced tools like dPCR, farmers have a powerful toolkit to keep their flocks safe and productive.

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What’s New in PCR Tech?

Both qPCR and dPCR are evolving fast. For qPCR, advancements like high-resolution melting analysis and integration with next-gen sequencing are making it more efficient and user-friendly. Meanwhile, dPCR is getting a high-tech boost with microfluidics and AI-driven data analysis, making it faster and more affordable.

These innovations are not just lab upgrades they’re becoming accessible tools for field veterinarians and farmers, ensuring that cutting-edge diagnostics reach those who need them most.

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A Future of Healthy Flocks and Sustainable Farming

The fight against poultry viruses is far from over, but with tools like dPCR and life-saving vaccines, we’re better equipped than ever. As diagnostics become smarter and prevention strategies stronger, the poultry industry can look forward to healthier flocks and more resilient farming practices.

By combining the best of technology and veterinary care, we’re not just protecting birds we’re securing the future of poultry farming for generations to come.

BIS Research provides a customized Go-to-Market Strategy specifically for the Healthcare-medical sector, offering expert guidance to help businesses explore and penetrate emerging markets and capitalize on new opportunities.

Conclusion

While quantitative PCR remains a reliable and efficient tool for detecting single pathogens, its limitations in handling complex co-infections make it less suited for modern veterinary challenges. On the other hand, digital PCR stands out as a superior technology, offering unparalleled accuracy, sensitivity, and the ability to detect multiple pathogens simultaneously. When combined with robust vaccination programs and ongoing advancements in both PCR technologies, these tools create a comprehensive approach to combating poultry diseases, ensuring healthier flocks and more sustainable farming practices.


 

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